Composite
Part:BBa_K3286103:Design
Designed by: Robert Hooftman Group: iGEM19_Wageningen_UR (2019-10-14)
Strep + MBP + tev site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 387
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 85
Design Notes
Amplify this fragment first with primers containing restriction enzyme site.
Source
PCR from plasmid