Composite

Part:BBa_K3286103:Design

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-14)


Strep + MBP + tev site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 85


Design Notes

Amplify this fragment first with primers containing restriction enzyme site.


Source

PCR from plasmid

References